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promoter into pBBR1MCS4, yielding pBBRN66. This plasmid was then introduced to the corresponding mutant PAN66 by electroporation.
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mutants, it absolutely was proposed that kind IV pili and flagella, two organelles of motility, may possibly Enjoy a very important purpose through the Preliminary interaction from the bacterial cell While using the area by counteracting repulsive forces. Also, style IV pili appeared to be essential with the initial differentiation by selling cell aggregation as well as the formation of microcolonies.
By analyzing the PAO1 genome, we could identify two more gene clusters encoding an entire list of factors belonging to your chaperone/usher pathway. The cupB
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I, and religated, and the round DNA was utilized as matrix for PCR amplification. The DNA fragments As a result amplified were being immediately cloned in to the PCR2.1 vector Along with the TA cloning package (Invitrogen) and remodeled into E. coli
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phenotype of the PAN66 mutant pressure was complemented from the introduction of the one of a kind gene. The dearth of attachment with the mutant PAN66 was consequently due entirely towards the disruption of your ladN66
The DNA sequences acquired were being analyzed with the blastn method and in comparison Together with the PAO1 genome at . The putative ORFs have been then analyzed with ORF finder (). The annotated genome was utilized on launch to update our Investigation.
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